Two component fitting of Nitroxide spins
Posted: Sun Jul 10, 2016 5:45 am
Hi all,
Im trying to fit a two component spectrum of an MTSL spin labelled protein. I use a first genetic fitting followed by a levenberg marquardt fitting. The overall result that im getting seems reasonable however the fitting of the broad components of the spectra seems to be out - which is also the region the spectra differ the most and the region im most interested (see attached). Did some have similar issues and could overcome these to achieve an better overall fit? Thanks for any help!
Im trying to fit a two component spectrum of an MTSL spin labelled protein. I use a first genetic fitting followed by a levenberg marquardt fitting. The overall result that im getting seems reasonable however the fitting of the broad components of the spectra seems to be out - which is also the region the spectra differ the most and the region im most interested (see attached). Did some have similar issues and could overcome these to achieve an better overall fit? Thanks for any help!