Two component fitting of Nitroxide spins

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oettingen
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Posts: 1
Joined: Sun Jul 10, 2016 5:19 am

Two component fitting of Nitroxide spins

Post by oettingen »

Hi all,

Im trying to fit a two component spectrum of an MTSL spin labelled protein. I use a first genetic fitting followed by a levenberg marquardt fitting. The overall result that im getting seems reasonable however the fitting of the broad components of the spectra seems to be out - which is also the region the spectra differ the most and the region im most interested (see attached). Did some have similar issues and could overcome these to achieve an better overall fit? Thanks for any help!
Attachments
NItroxide_sim.png
NItroxide_sim.png (97.05 KiB) Viewed 6934 times
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